Fig 1C of the Replication Study: Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET uses an odd (to me) three stage normalization procedure for the quantified western blots. The authors compared blot values between a treatment (shMet cells) and a control (shScr cells) using GAPDH to normalize the values. The three stages of the normalization are
first, the value for the Antibody levels were normalized by the value of a reference (GAPDH) for each Set.
